In this vignette we walk through an example illustrating how **GenEst** command line utilities could be used to estimate mortality for different size birds at a large field of solar photovoltaic collectors. Our objective is to estimate overall mortality, as well as how mortality varies over time, whether it constant throughout the facility, and finally how different size classes of birds are affected.

The general steps in the analysis are:

- Construct a model for Searcher Efficiency
- Construct a model for Carcass Persistance
- Estimate mortality
- Specify the type of summary desired (for example, by season and species)

There are five files in total which make up the example dataset, located in `\GenEst\inst\extdata\solar_PV`

. For convenience, these files have been made available in R as a list,

Searcher efficiency (SE) is modeled as a function of the number of times a carcass has been missed in previous searches and any number of covariates. The probability of finding a carcass that is present at the time of search is p on the first search after carcass arrival and is assumed to decrease by a factor of k each time the carcass is missed in searches. (For further background on field trials, and information about how to format the results for use with GenEst, see the User Guide).

Results of the SE field trials used in this example are stored in the `data_SE`

data frame:

```
data_SE <- solar_PV$SE
head(data_SE)
#> seID Season Size Search1 Search2 Search3 Search4 Distance
#> 1 se1 winter small 1 NA NA NA 17
#> 2 se2 winter small 1 NA NA NA 26
#> 3 se3 winter small 0 1 NA NA 54
#> 4 se4 winter small 0 1 NA NA 28
#> 5 se5 winter small 1 NA NA NA 46
#> 6 se6 winter small 1 NA NA NA 40
```

GenEst provides tools to construct and compare specific individual models, to explore which subsets of variables are most useful, and to automatically construct entire sets of models. To start we will fit a basic model in which the probability of detecting a carcass, `p`

, and compounding difficulty to detect, `k`

, depend only on their respective intercepts (and not other factors such as season or size). The function `pkm`

is used to create a searcher efficiency model, which is returned as a `pkm`

object.

```
SE_model <- pkm(p ~ 1, k ~ 1, data = data_SE)
SE_model
#> $call
#> pkm0(formula_p = formula_p, formula_k = formula_k, data = data,
#> obsCol = obsCol, kFixed = kFixed, kInit = kInit, CL = CL,
#> quiet = quiet)
#>
#> $formula_p
#> p ~ 1
#>
#> $formula_k
#> k ~ 1
#>
#> $predictors
#> character(0)
#>
#> $AICc
#> [1] 489
#>
#> $convergence
#> [1] 0
#>
#> $cell_pk
#> cell n p_median p_lower p_upper k_median k_lower k_upper
#> 1 all 240 0.665 0.615 0.711 0.846 0.742 0.913
#>
#> $CL
#> [1] 0.9
```

To explore whether use of covariate is warranted, `pkm`

is used with the `allCombos = TRUE`

. The specified model will be fit as will models formed using all combinations of predictors listed for the `p`

and `k`

parameters. orginal model. For example, `p ~ Season`

can be simplified into `p ~ 1`

, our original model in which `p`

is independent of season.

```
SE_model_set <- pkm(p~Season, k~1, data = data_SE, allCombos = TRUE)
class(SE_model_set)
#> [1] "pkmSet" "list"
length(SE_model_set)
#> [1] 2
names(SE_model_set)
#> [1] "p ~ Season; k ~ 1" "p ~ 1; k ~ 1"
class(SE_model_set[[1]])
#> [1] "pkm" "list"
```

The set of models is contained in a `pkmSet`

object. We could inspect the two models stored in the `pkmSet`

individually, or for convenience we can view the AICc values simultaneously for all models using the function. Summary plots can be obtained by plotting any of the individual objects or the set as well.

```
aicc(SE_model_set)
#> p Formula k Formula AICc <U+0394>AICc
#> 2 p ~ 1 k ~ 1 489.00 0.00
#> 1 p ~ Season k ~ 1 492.11 3.11
```

Rather than one searcher efficiency model for all birds, it is often preferable to fit a seperate model for each size class. The `sizeCol`

argument of the `pkm`

function is the name of the column in `data_SE`

that gives the size class for each carcass in the SE trials. If a `sizeCol`

is provided, `pkm`

returns a list of separate pk models fit for each size class.

```
SE_size_model <- pkm(p ~ Season,
k ~ 1,
sizeCol = "Size",
data = data_SE)
class(SE_size_model)
#> [1] "pkmSize" "list"
names(SE_size_model) # A list is created with a model set per size class.
#> [1] "lrg" "med" "small"
class(SE_size_model$small)
#> [1] "pkm" "list"
names(SE_size_model$small) # Each model set contains one model in this case.
#> [1] "call" "data" "data0" "formula_p"
#> [5] "formula_k" "predictors" "predictors_p" "predictors_k"
#> [9] "AIC" "AICc" "convergence" "varbeta"
#> [13] "cellMM_p" "cellMM_k" "nbeta_p" "nbeta_k"
#> [17] "betahat_p" "betahat_k" "levels_p" "cells"
#> [21] "ncell" "cell_pk" "CL" "observations"
#> [25] "carcCells" "loglik" "pOnly"
```

To fit all combinations of models for each size class, use `pkm`

with a `sizeCol`

parameter and with `allCombos = T`

.

Once we have decided on which models to use for each size class, we store the corresponding pkm objects in a list for future use. In this case, we will choose the models with the lower AICc.

```
SE_size_model_set <- pkm(p ~ Season,
k ~ 1,
sizeCol = "Size",
data = data_SE, allCombos = TRUE)
aicc(SE_size_model_set)
#> $lrg
#> p Formula k Formula AICc <U+0394>AICc
#> 1 p ~ Season k ~ 1 95.65 0.00
#> 2 p ~ 1 k ~ 1 96.47 0.82
#>
#> $med
#> p Formula k Formula AICc <U+0394>AICc
#> 2 p ~ 1 k ~ 1 178.62 0.00
#> 1 p ~ Season k ~ 1 182.40 3.78
#>
#> $small
#> p Formula k Formula AICc <U+0394>AICc
#> 2 p ~ 1 k ~ 1 202.17 0.00
#> 1 p ~ Season k ~ 1 206.48 4.31
SE_models <- list()
```

Size small:

Size Medium:

Size Large:

A carcass persistence model estimates the amount of time a carcass would persist for, given the conditions under which it arrived. A number of carcasses have been placed in the field and periodically checked for scavanging. Results of the CP field trials used in this example are stored in the `data_CP`

data frame:

```
data_CP <- solar_PV$CP
head(data_CP)
#> cpID Season Size LastPresent FirstAbsent
#> 1 cp1 winter small 0.00 1.05
#> 2 cp2 winter small 21.02 27.97
#> 3 cp3 winter small 4.03 7.01
#> 4 cp4 winter small 0.00 0.97
#> 5 cp5 winter small 27.99 NA
#> 6 cp6 winter small 10.02 13.99
```

and represent the left (start) and right (end) endpoints of the interval over which a carcass went missing. For further information about CP trials and how to format results for use with GenEst, see the User Guide (link found on help menu of the GUI, which can be accessed by entering `runGenEst()`

from the R console).

Four classes of parameteric models may be used for carcass persistance: exponential, Weibull, logistic, and lognormal. As with Searcher Efficiency we can fit one specific model, test a set of covariates and choose our favorite single model, or fit seperate models dependent on size class. First we will fit a single Weibull models for all birds. Weibull distributions have two parameters, location and scale. We will specify that the location depends on season by setting `l ~ season`

, but scale only depends on the intercept using `s ~ 1`

.

```
cpm(l ~ Season, s ~ 1, data = data_CP,
left = "LastPresent",
right = "FirstAbsent",
dist = "weibull")
#> $call
#> cpm0(formula_l = formula_l, formula_s = formula_s, data = data,
#> left = left, right = right, dist = dist, CL = CL, quiet = quiet)
#>
#> $formula_l
#> l ~ Season
#>
#> $formula_s
#> s ~ 1
#>
#> $distribution
#> [1] "weibull"
#>
#> $predictors
#> [1] "Season"
#>
#> $AICc
#> [1] 1051.61
#>
#> $convergence
#> [1] 0
#>
#> $cell_ls
#> cell n l_median l_lower l_upper s_median s_lower s_upper
#> 1 fall 60 2.473 2.087 2.859 1.637 1.457 1.838
#> 2 spring 60 2.801 2.395 3.208 1.637 1.457 1.838
#> 3 summer 60 2.689 2.296 3.083 1.637 1.457 1.838
#> 4 winter 60 2.509 2.127 2.891 1.637 1.457 1.838
#>
#> $cell_ab
#> cell n pda_median pda_lower pda_upper pdb_median pdb_lower pdb_upper
#> 1 fall 60 0.611 0.686 0.544 11.858 8.061 17.444
#> 2 spring 60 0.611 0.686 0.544 16.461 10.968 24.730
#> 3 summer 60 0.611 0.686 0.544 14.717 9.934 21.824
#> 4 winter 60 0.611 0.686 0.544 12.293 8.390 18.011
#>
#> $CL
#> [1] 0.9
#>
#> $cell_desc
#> cell medianCP r1 r3 r7 r14 r28
#> 1 fall 6.508736 0.8733664 0.7695707 0.6489244 0.5240883 0.3856577
#> 2 spring 9.035276 0.8948948 0.8063180 0.7000989 0.5856841 0.4518313
#> 3 summer 8.078012 0.8879564 0.7943671 0.6832539 0.5651042 0.4292548
#> 4 winter 6.747503 0.8759150 0.7738696 0.6548159 0.5310385 0.3929133
```

Next, we try a CP model set considering whether the `season`

covariate for location is necessary, by comparing the `l ~ season, s ~ 1`

to `l ~ 1, s ~ 1`

.

```
CP_weibull_set <- cpm(l ~ Season, s ~ 1, data = data_CP,
left = "LastPresent",
right = "FirstAbsent",
dist = "weibull", allCombos = TRUE)
class(CP_weibull_set)
#> [1] "cpmSet" "list"
aicc(CP_weibull_set)
#> Distribution Location Formula Scale Formula AICc <U+0394>AICc
#> 2 weibull l ~ 1 s ~ 1 1046.65 0.00
#> 1 weibull l ~ Season s ~ 1 1051.61 4.96
```

Finally we will construct sets of CP models for each size class, however this time we will also consider models based on both exponential and weibull distributions. To compare models for multiple distributions, set `dist`

to a vector of the distribution names to be considered. With a `sizeCol`

provided and `allCombos = TRUE`

, `cpm`

returns a list of `cpmSet`

objects, one for each size class.

```
CP_size_model_set <- cpm(formula_l = l ~ Season,
formula_s = s ~ 1,
left = "LastPresent",
right = "FirstAbsent",
dist = c("exponential", "weibull"),
sizeCol = "Size",
data = data_CP, allCombos = TRUE)
class(CP_size_model_set)
#> [1] "cpmSetSize" "list"
names(CP_size_model_set)
#> [1] "lrg" "med" "small"
class(CP_size_model_set$small)
#> [1] "cpmSet" "list"
length(CP_size_model_set$small)
#> [1] 4
names(CP_size_model_set$small)
#> [1] "dist: exponential; l ~ Season; NULL"
#> [2] "dist: exponential; l ~ 1; NULL"
#> [3] "dist: weibull; l ~ Season; s ~ 1"
#> [4] "dist: weibull; l ~ 1; s ~ 1"
```

We now have the flexibility to select models from different families for different size classes. We will choose to use the models with lower AICc, which requires storing the corresponding `cpm`

objects in a list for later use.

```
aicc(CP_size_model_set)
#> $lrg
#> Distribution Location Formula Scale Formula AICc <U+0394>AICc
#> 2 exponential l ~ 1 NULL 294.59 0.00
#> 4 weibull l ~ 1 s ~ 1 295.60 1.01
#> 1 exponential l ~ Season NULL 299.61 5.02
#> 3 weibull l ~ Season s ~ 1 300.86 6.27
#>
#> $med
#> Distribution Location Formula Scale Formula AICc <U+0394>AICc
#> 4 weibull l ~ 1 s ~ 1 352.44 0.00
#> 3 weibull l ~ Season s ~ 1 356.47 4.03
#> 2 exponential l ~ 1 NULL 358.25 5.81
#> 1 exponential l ~ Season NULL 361.45 9.01
#>
#> $small
#> Distribution Location Formula Scale Formula AICc <U+0394>AICc
#> 4 weibull l ~ 1 s ~ 1 346.02 0.00
#> 3 weibull l ~ Season s ~ 1 348.88 2.86
#> 1 exponential l ~ Season NULL 380.56 34.54
#> 2 exponential l ~ 1 NULL 382.04 36.02
CP_models <- list()
```

Size small:

Size med:

Size lrg:

Estimating mortality requires bringing together models, carcass observation data (CO), and information on how the data was gathered. In particular the search schedule (SS) and proportion of carcasses in searchable areas (the density weighted proportion, or DWP), are needed. We will breifly inspect the files. Further information on the formatting of the CO, SS, and DWP files can be found in the User Guide.

Carcass observations:

```
data_CO <- solar_PV$CO
head(data_CO)
#> carcID Unit Species Size Row Distance DateFound X Y
#> 1 x1 Unit82 SA small 28 13 2018-01-26 524641 3857110
#> 2 x2 Unit147 SA small 30 23 2018-01-13 525440 3857592
#> 3 x3 Unit235 SA small 11 44 2018-03-18 526724 3858148
#> 4 x4 Unit213 SA small 31 44 2018-02-04 526408 3858069
#> 5 x5 Unit204 SA small 34 10 2018-04-29 524953 3858081
#> 6 x6 Unit117 SA small 25 27 2018-04-06 527023 3857256
```

Search schedule:

```
data_SS <- solar_PV$SS
data_SS[1:5 , 1:10]
#> DateSearched Season Unit1 Unit2 Unit3 Unit4 Unit5 Unit6 Unit7 Unit8
#> 1 2017-12-20 winter 1 1 1 1 1 1 1 1
#> 2 2017-12-21 winter 1 1 1 1 1 1 1 1
#> 3 2017-12-22 winter 0 0 0 0 0 0 0 0
#> 4 2017-12-23 winter 0 0 0 0 0 0 0 0
#> 5 2017-12-24 winter 0 0 0 0 0 0 0 0
```

(Note that there are 300 arrays columns altogether: Unit1, …, Unit300)

Density weighted proportion:

```
data_DWP <- solar_PV$DWP
head(data_DWP)
#> Unit small med lrg
#> 1 Unit1 1 1 1
#> 2 Unit2 1 1 1
#> 3 Unit3 1 1 1
#> 4 Unit4 1 1 1
#> 5 Unit5 1 1 1
#> 6 Unit6 1 1 1
```

These elements combine in the function `estM`

, producing an object containing simulated arrival, detection, and mortality distributions. We also have the opportunity to provide the fraction of the facility being surveyed, `frac`

, if it happens to be less than 100%. Increasing the number of simulations, `nsim`

, will improve the accuracy of the estimates but comes at a cost of computer runtime.

When estimating mortality, it is not currently possible to mix CP and SE models which differ in their dependence on size. Either both models depend on size class, or both models must be independent of size class. In this case we will choose here to use size dependence.

```
Mest <- estM(
nsim = 100, frac = 1,
data_CO = data_CO, data_SS = data_SS, data_DWP = data_DWP,
model_SE = SE_models, model_CP = CP_models,
unitCol = "Unit", sizeCol = "Size",
COdate = "DateFound", SSdate = "DateSearched"
)
```

We are now able to get a confidence interval for estimated total mortality by taking summary of the estM object. Plotting it shows us us the estimated probability density for number of fatalities.

A point estimate for overall sitewide mortality is listed at the top of the plot, satisfying our first objective. The period of inference only covers the period over which we have have fatality monitoring data, which in this case is from 2017-12-20 to 2018-12-19.

Having calculated the estimated arrival densities for each of the carcases, we can now use them to create a variety of summaries. Suppose that we are interested in how mortality changes with respect to three kinds of variables:

- Temporally - by season, or finer resolutions
- Spatially - summaries by search unit
- Among Size classes, or other groups

To create summaries, we split the data by differnt covariates, using a function called `calcSplits`

. This requires the simulated mortality `$Mhat`

and arrival times `$Aj`

stored in the `estM`

object, plus the search schedule and carcass observation data.

Splits to the search schedule (splits in time) are specified by assigning a covariate to `split_SS`

. These must be variables present in the Search Schedule file. To investigate differences in mortality between season, we will set `split_SS`

to `Season`

.

```
unique(data_SS[, "Season"])
#> [1] "winter" "spring" "summer" "fall"
M_season <- calcSplits(M = Mest,
split_SS = "Season", data_SS = data_SS,
split_CO = NULL, data_CO = data_CO
)
```

Splitting the estM creates a `splitFull`

object, a plot of which shows boxplots for each season.

Taking a summary of the `splitFull`

object gives us a confidence interval for each level of the split covariate. The size of the confidence interval can be specified for both plots or summaries using the CL argument.

```
summary(M_season, CL = 0.95)
#> X 2.5% 25% 50% 75% 97.5%
#> winter 29.00714 38.05846 49.49365 54.52377 61.10987 73.94128
#> spring 84.86714 157.96974 178.81541 192.03429 209.24632 246.24679
#> summer 73.59286 126.54543 146.20493 157.10979 169.47047 201.09202
#> fall 45.53286 79.45912 90.08265 98.99051 107.63586 128.05754
#> attr(,"class")
#> [1] "splitSummary"
#> attr(,"CL")
#> [1] 0.95
#> attr(,"vars")
#> [1] "Season"
#> attr(,"type")
#> [1] "SS"
#> attr(,"times")
#> [1] 0 89 182 276 364
```

To get a finer summary of mortality, we need to parse the search schedule, using the function `prepSS`

. This allows us to specify the exact time intervals over which we will split, in this case we will create a weekly summary.

```
SSdat <- prepSS(data_SS) # Creates an object of type prepSS.
schedule <- seq(from = 0, to = max(SSdat$days), by = 7)
tail(schedule)
#> [1] 329 336 343 350 357 364
```

When we plot the splitFull object for a split with a custom schedule, we must specify that the rate is per split catagory by setting `rate = T`

.

```
M_week <- calcSplits(M = Mest,
split_time = schedule,
data_SS = SSdat,
data_CO = data_CO
)
plot(x = M_week, rate = TRUE)
```

Next we will look at at splitting by covariates present in the Carcass Observation file. We specify a CO split by assigning split_CO to the name (or names) of the variables we wish to split on. Suppose we would like a summary of estimated mortality by unit.

```
M_unit <- calcSplits(M = Mest,
split_CO = "Unit",
data_CO = data_CO,
data_SS = data_SS
)
plot(M_unit, rate = FALSE)
```

There are 300 units in this example, each one gets a boxplot when we plot the splitFull. For those arrays which have at least one observation, we can create a summary. In this case we will only create a summary for arrays 8 and 100.

```
dim(summary(M_unit)) # only 164 arrays had observations.
#> [1] 164 6
# A list of the arrays without observed carcasses:
setdiff(paste0("Unit", 1:300), data_CO$Unit)
#> [1] "Unit2" "Unit3" "Unit4" "Unit5" "Unit9" "Unit13" "Unit17"
#> [8] "Unit19" "Unit20" "Unit24" "Unit25" "Unit28" "Unit29" "Unit34"
#> [15] "Unit35" "Unit36" "Unit37" "Unit38" "Unit39" "Unit40" "Unit41"
#> [22] "Unit42" "Unit45" "Unit47" "Unit48" "Unit51" "Unit55" "Unit56"
#> [29] "Unit57" "Unit59" "Unit60" "Unit62" "Unit63" "Unit66" "Unit69"
#> [36] "Unit70" "Unit71" "Unit73" "Unit74" "Unit75" "Unit81" "Unit84"
#> [43] "Unit85" "Unit86" "Unit88" "Unit90" "Unit91" "Unit93" "Unit94"
#> [50] "Unit95" "Unit97" "Unit99" "Unit102" "Unit103" "Unit104" "Unit105"
#> [57] "Unit108" "Unit109" "Unit111" "Unit119" "Unit120" "Unit121" "Unit122"
#> [64] "Unit124" "Unit125" "Unit127" "Unit128" "Unit132" "Unit133" "Unit136"
#> [71] "Unit143" "Unit146" "Unit148" "Unit149" "Unit151" "Unit164" "Unit166"
#> [78] "Unit169" "Unit172" "Unit175" "Unit179" "Unit181" "Unit185" "Unit186"
#> [85] "Unit188" "Unit189" "Unit191" "Unit192" "Unit193" "Unit197" "Unit199"
#> [92] "Unit200" "Unit201" "Unit202" "Unit205" "Unit209" "Unit211" "Unit216"
#> [99] "Unit221" "Unit222" "Unit225" "Unit226" "Unit228" "Unit231" "Unit233"
#> [106] "Unit234" "Unit236" "Unit246" "Unit247" "Unit249" "Unit251" "Unit254"
#> [113] "Unit255" "Unit256" "Unit257" "Unit259" "Unit261" "Unit262" "Unit264"
#> [120] "Unit266" "Unit267" "Unit272" "Unit274" "Unit275" "Unit280" "Unit282"
#> [127] "Unit283" "Unit285" "Unit288" "Unit289" "Unit291" "Unit293" "Unit294"
#> [134] "Unit295" "Unit297" "Unit298"
# Create summaries for arrays Unit12 and Unit100.
whichRow <- rownames(summary(M_unit)) %in% c("Unit12", "Unit100")
summary(M_unit)[whichRow, ]
#> X 5% 25% 50% 75% 95%
#> Unit12 1 1 1.025620 1.963654 3.545283 5.172705
#> Unit100 3 3 4.638041 6.085100 7.325331 10.153726
```

It is possible to create summaries that split on both Carcass Observation variables and Search Schedule variables. To do so, include both a `split_SS`

and a `split_CO`

argument.

```
M_unit_and_species <- calcSplits(M = Mest,
split_SS = c("Season"),
split_CO = c("Size"),
data_CO = data_CO,
data_SS = data_SS
)
plot(M_unit_and_species, rate = FALSE)
```

Two CO variables can be compared simultaneously by specifying an ordered pair of covariates for `split_CO`

, however currently there are a limited total number (two) of splits which can be allocated among temporal or carcass covariates.