crispRdesignR: Guide Sequence Design for CRISPR/Cas9

Designs guide sequences for CRISPR/Cas9 genome editing and provides information on sequence features pertinent to guide efficiency. Sequence features include annotated off-target predictions in a user-selected genome and a predicted efficiency score based on the model described in Doench et al. (2016) <doi:10.1038/nbt.3437>. Users are able to import additional genomes and genome annotation files to use when searching and annotating off-target hits. All guide sequences and off-target data can be generated through the 'R' console with sgRNA_Design() or through 'crispRdesignR's' user interface with crispRdesignRUI(). CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and the associated protein Cas9 refer to a technique used in genome editing.

Version: 1.1.7
Depends: R (≥ 2.10)
Imports: Biostrings, gbm, GenomicRanges, BiocGenerics, IRanges, GenomeInfoDb, S4Vectors, rtracklayer, stringr, vtreat, shiny, DT
Suggests: BSgenome.Scerevisiae.UCSC.sacCer3
Published: 2023-04-12
DOI: 10.32614/CRAN.package.crispRdesignR
Author: Dylan Beeber [aut, cre], Frederic Chain [aut]
Maintainer: Dylan Beeber <dylan.beeber at>
License: GPL-3
URL: <>
NeedsCompilation: no
Materials: README
CRAN checks: crispRdesignR results


Reference manual: crispRdesignR.pdf


Package source: crispRdesignR_1.1.7.tar.gz
Windows binaries: r-devel:, r-release:, r-oldrel:
macOS binaries: r-release (arm64): crispRdesignR_1.1.7.tgz, r-oldrel (arm64): crispRdesignR_1.1.7.tgz, r-release (x86_64): crispRdesignR_1.1.7.tgz, r-oldrel (x86_64): crispRdesignR_1.1.7.tgz
Old sources: crispRdesignR archive


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