Inverse estimation, also referred to as the calibration problem, is a classical and well-known problem in regression. In simple terms, it involves the use of an observed value of the response (or specified value of the mean response) to make inference on the corresponding unknown value of the explanatory variable.
A detailed introduction to investr has been published in The R Journal: "investr: An R Package for Inverse Estimation", http://journal.r-project.org/archive/2014-1/greenwell-kabban.pdf. You can track development at https://github.com/bgreenwell/investr. To report bugs or issues, contact the main author directly or submit them to https://github.com/bgreenwell/investr/issues.
As of right now,
investr supports (univariate) inverse estimation with objects of class:
lm- linear models (multiple predictor variables allowed)
glm- generalized linear models (multiple predictor variables allowed)
nls- nonlinear least-squares models
lme- linear mixed-effects models (fit using the
The package is currently listed on CRAN and can easily be installed:
# Install from CRAN install.packages("investr", dep = TRUE)
The package is also part of the ChemPhys task view, a collection of R packages useful for analyzing data from chemistry and physics experiments. These packages can all be installed at once (including
investr) using the
ctv package (Zeileis, 2005):
# Install the ChemPhys task view install.packages("ctv") ctv::install.views("ChemPhys")
In binomial regression, the estimated lethal dose corresponding to a specific probability p of death is often referred to as LDp.
invest obtains an estimate of LDp by inverting the fitted mean response on the link scale. Similarly, a confidence interval for LDp can be obtained by inverting a confidence interval for the mean response on the link scale.
library(investr) # Dobson's beetle data head(beetle) # Binomial regression binom_fit <- glm(cbind(y, n-y) ~ ldose, data = beetle, family = binomial(link = "cloglog")) plotFit(binom_fit, lwd.fit = 2, cex = 1.2, pch = 21, bg = "lightskyblue", lwd = 2, xlab = "Log dose", ylab = "Probability") # Inverse estimation invest(binom_fit, y0 = 0.5) # median lethal dose invest(binom_fit, y0 = 0.9) # 90% lethal dose invest(binom_fit, y0 = 0.99) # 99% lethal dose # estimate lower upper # 1.7788 1.7702 1.7862
To obtain an estimate of the standard error, we can use the Wald method:
invest(binom_fit, y0 = 0.5, interval = "Wald") # estimate lower upper se # 1.7788 1.7709 1.7866 0.0040 # The MASS package function dose.p works too MASS::dose.p(binom_fit, p = 0.5) # Dose SE # p = 0.5: 1.778753 0.00400654
Multiple predictor variables are allowed for objects of class
glm. For instance, the example from
?MASS::dose.p can be re-created as follows:
# Load package, assuming it is already installed library(MASS) # Data ldose <- rep(0:5, 2) numdead <- c(1, 4, 9, 13, 18, 20, 0, 2, 6, 10, 12, 16) sex <- factor(rep(c("M", "F"), c(6, 6))) SF <- cbind(numdead, numalive = 20 - numdead) budworm <- data.frame(ldose, numdead, sex, SF) # Logistic regression budworm.lg0 <- glm(SF ~ sex + ldose - 1, family = binomial, data = budworm) # Using dose.p function from package MASS dose.p(budworm.lg0, cf = c(1, 3), p = 1/4) # Dose SE # p = 0.25: 2.231265 0.2499089 # Using invest function from package investr invest(budworm.lg0, y0 = 1/4, interval = "Wald", x0.name = "ldose", newdata = data.frame(sex = "F")) # estimate lower upper se # 2.2313 1.7415 2.7211 0.2499
The data here contain the actual concentrations of an agrochemical present in soil samples versus the weight of the plant after three weeks of growth. These data are stored in the data frame
nasturtium and are loaded with the package. A simple log-logistic model describes the data well:
# Log-logistic model log_fit <- nls(weight ~ theta1/(1 + exp(theta2 + theta3 * log(conc))), start = list(theta1 = 1000, theta2 = -1, theta3 = 1), data = nasturtium) plotFit(log_fit, lwd.fit = 2)
Three new replicates of the response (309, 296, 419) at an unknown concentration of interest (x0) are measured. It is desired to estimate x0.
# Inversion method invest(log_fit, y0 = c(309, 296, 419), interval = "inversion") # estimate lower upper # 2.2639 1.7722 2.9694 # Wald method invest(log_fit, y0 = c(309, 296, 419), interval = "Wald") # estimate lower upper se # 2.2639 1.6889 2.8388 0.2847
The intervals both rely on large sample results and normality. In practice, the bootstrap may be more reliable:
# Bootstrap calibration intervals (may take a few seconds) boo <- invest(log_fit, y0 = c(309, 296, 419), interval = "percentile", nsim = 9999, seed = 101, progress = TRUE) boo # print bootstrap summary # estimate lower upper se bias # 2.2639 1.7890 2.9380 0.2947 0.0281 plot(boo) # plot results