Dowser is part of the Immcantation analysis framework for Adaptive Immune Receptor Repertoire sequencing (AIRR-seq). Dowser provides a set of tools for performing phylogenetic analysis on B cell receptor repertoires. It supports building and visualizing trees using multiple methods, and implements statistical tests for discrete trait analysis of B cell migration, differentiation, and isotype switching.

Dowser has 6 primary functions:

  1. Reconstruct clonal germline sequences.
  2. Build B cell lineage trees using multiple methods, such as maximum parsimony, maximum likelihood, and IgPhyML.
  3. Reconstruct intermediate sequences within lineage trees using different methods.
  4. Create publication-quality lineage tree plots.
  5. Analyze trees to detect ongoing B cell evolution over time.
  6. Understand B cell migration and differentiation.

What’s a dowser?


For help and questions please contact the Immcantation Group or use the issue tracker.